Hot Spot Determination

Identifying a specific subset of key amino acids in a protein or protein system is critical to determining its functional characteristic and overall stability. These amino acid residues play a role either independently or as part of a cluster of networked residues, resulting in long-range effects of distal mutations. ZymeCAD™ comprises proprietary algorithms that identify these critical residues (referred to as ‘hot spots’) based on characterization of residue properties in simulated trajectories of the protein and assess the mutability of specific residues based on its flexibility profile and local environment.

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